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Image Search Results
Journal: Cell Death & Disease
Article Title: Oxidative stress-induced mitophagy is suppressed by the miR-106b-93-25 cluster in a protective manner
doi: 10.1038/s41419-021-03484-3
Figure Lengend Snippet: A, C, D EGFP-Parkin-transfected and EGFP-C1-transfected HeLa cells were stimulated with 100 μΜ H 2 O 2 for 0 h or 12 h and stained with anti-Myc, anti-Myc-S 62 , and anti-Myc-T 58 antibodies (red). Scale bars, 10 μm. B , E , F Cells were transfected with EGFP-C1 or EGFP-Parkin plasmids. After 24 h, the cells were treated with 100 μΜ H 2 O 2 for 0 h or 12 h. Nuclear extracts were immunoblotted for c-Myc, p-c-Myc-S62, and p-c-Myc-T58. Lamin B1 was used as a nuclear internal reference, and Tubulin was used as a cytoplasmic internal reference. N = 3; * P < 0.05; ** P < 0.01. The data are from three independent tests and are presented as the mean ± SD.
Article Snippet: The antibodies used for WB and IF included TFAM (WB 1:1 000, IF 1:200, Proteintech, 23996-1-AP), AMBRA1 (WB 1:500, IF 1:100, Abclonal, A1083), MCU (WB 1:1 000, Proteintech, 26312-1-AP), NRF2 (WB 1:1 000, IF 1:200, Proteintech, 16396-1-AP), Beta Tubulin (WB 1:40 000, Proteintech, 66240-1-Ig), CASP3 (WB 1:1 000, Abclonal, A2156), PP2A-Cα/β (WB 1:1 000, IF 1:200, Santa Cruz, SC-80665) GSK3β (WB 1:1 000, IF 1:200, Abclonal, A2081), p-GSK3β-S9 (IF 1:200, Proteintech, 67558-1-Ig), p-c-Myc-S62 (WB 1:1 000, IF 1:200, Abcam, AB185656), AXIN1 (WB 1:1 000, Abclonal, A16019), TOMM20 (WB 1:1 000, IF 1:200, Abclonal, A16896),
Techniques: Transfection, Staining
Journal: Microbiology Spectrum
Article Title: Mandarin Fish ( Siniperca chuatsi ) p53 Regulates Glutaminolysis Induced by Virus via the p53/miR145-5p/c-Myc Pathway in Chinese Perch Brain Cells
doi: 10.1128/spectrum.02727-21
Figure Lengend Snippet: Effects of regulating the Sc-p53/miR145-5p/c-Myc pathway on GLS1. (A and B) The mRNA expression (A) and protein expression (B) of p53, miR145-5p, c-Myc, and GLS1 in CPB cells treated with PFT-α or 5-Fu. (C and D) mRNA (C) and protein (D) expression of c-Myc and GLS1 in CPB cells treated with mimics or inhibitor of miR145-5p. (E and F) mRNA (E) and protein (F) expression of c-Myc and GLS1 in CPB cells transfected with NC small interfering RNA (siRNA) or c-Myc siRNA (si-c-Myc) or pCMV-EGFP vector (p-vector) or pCMV-EGFP-Sc-c-Myc overexpression vector (p-c-Myc). (G and H) mRNA (G) and protein (H) expression of GLS1 in miR145-5p inhibitor-transfected cells treated with PFT-α or 5-Fu. β-Actin was used as a control for qRT-PCR and Western blotting analysis. Asterisks indicate the significant differences from the control group (*, P < 0.05; **, P < 0.01; ***, P < 0.001).
Article Snippet: The
Techniques: Expressing, Transfection, Small Interfering RNA, Plasmid Preparation, Over Expression, Control, Quantitative RT-PCR, Western Blot